Introduction and application of CAS:9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
3 -hydroxysteroid dehydrogenase is a steroid dehydrogenase secreted by Comamonas testosteroni. It consists of 258 amino acid residues and has a theoretical molecular weight of 26.4kD. 3 -hydroxysteroid dehydrogenase can act on a variety of steroid substrates and reversibly catalyze the oxidation and reduction of the hydroxyl/keto group at the 3-position of C19-27 steroids. The isoelectric point of HSD is 4.8, and the optimal pH is 7-9. Hg2+, Ag+, etc. will inhibit enzyme activity. Metal ion chelating agents such as EDTA will not inhibit enzyme activity and are helpful for the stable preservation of enzymes to a certain extent. 3 -hydroxysteroid dehydrogenase is a white yellowish amorphous powder. 3 -hydroxysteroid dehydrogenase is a white yellowish amorphous powder. Bile acid is one of the substrates of 3 -hydroxysteroid dehydrogenase. Clinically 3 -hydroxysteroid dehydrogenase is used to catalyze the dehydrogenation reaction of hydroxysteroids for clinical detection of total bile acids.
Specification of CAS:9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
HSD; 3 -Hydroxysteroid Dehydrogenase from microorganism; 3 -Hydroxysteroid:NAD(P)+ oxidoreductase
CAS number: 9028-56-2
Molecular weight: 28KDa (SDS-PAGE)
Vitality: Greater than or equal to 50units/mg protein
Activity definition: One unit will oxidize one micromole androsterone per min at pH 8.9 at 25 degree
Protein: Greater than or equal to 25%
Isoelectic point: 7.5
PH stability: 4.5-10.5
PH: 10.5
Temperature: 60 degree
<50℃ (pH7.2, 20min)
Inhibitors: Ag+, Hg2+
Preparation instructions: The enzyme is reconstituted in 30mM potassium phosphate buffer,PH7.2 for activity assay.
Storage: -20 degree
Package of CAS:9028-56-2|3ALPHA-HYDROXYSTEROID DEHYDROGENASE
1kg/drum
This product is valid for 24 months from the production date when stored in a warm sealed state.
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